HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Ion mobility resolved photo-fragmentation to discriminate protomers

Abstract : Rationale: Among the sources of structural diversity in biomolecular ions, the coexistence of protomers is particularly difficult to take into account, which in turn complicates structural interpretation of gas-phase data. Methods: We investigated the sensitivity of gas-phase photo-fragmentation measurements and ion mobility spectrometry (IMS) to the protonation state of a model peptide derivatized with chromophores. Accessible interconversion pathways between the different identified conformers were probed by tandem ion mobility measurement. Furthermore, the excitation coupling between the chromophores has been probed through photo-fragmentation measurements on mobility-selected ions. All results were interpreted based on molecular dynamics simulations. Results: We show that protonation can significantly affect the photo-fragmentation yields. Especially, conformers with very close collision cross sections (CCSs) may display dramatically different photo-fragmentation yields in relation with different protonation patterns. Conclusions: We show that, even if precise structure assignment based on molecular modeling is in principle difficult for large biomolecular assemblies, the combination of photo-fragmentation and IMS can help to identify the signature of protomer coexistence for a population of biomolecular ions in the gas phase. Such spectroscopic data are particularly suitable to follow conformational changes.
Complete list of metadata

Contributor : Philippe Dugourd Connect in order to contact the contributor
Submitted on : Monday, January 17, 2022 - 4:15:22 PM
Last modification on : Tuesday, January 18, 2022 - 8:53:10 AM
Long-term archiving on: : Monday, April 18, 2022 - 6:05:16 PM


Explicit agreement for this submission




Chang Min Choi, Alexander Kulesza, Steven Daly, Luke Macaleese, Rodolphe Antoine, et al.. Ion mobility resolved photo-fragmentation to discriminate protomers. Rapid Communications in Mass Spectrometry, Wiley, 2018, 33 (S1), pp.28-34. ⟨10.1002/rcm.8202⟩. ⟨hal-02108501⟩



Record views


Files downloads