HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Reinforcing one-carbon metabolism via folic acid/Folr1 promotes β-cell differentiation

Abstract : Abstract Diabetes can be caused by an insufficiency in β-cell mass. Here, we performed a genetic screen in a zebrafish model of β-cell loss to identify pathways promoting β-cell regeneration. We found that both folate receptor 1 ( folr1 ) overexpression and treatment with folinic acid, stimulated β-cell differentiation in zebrafish. Treatment with folinic acid also stimulated β-cell differentiation in cultures of neonatal pig islets, showing that the effect could be translated to a mammalian system. In both zebrafish and neonatal pig islets, the increased β-cell differentiation originated from ductal cells. Mechanistically, comparative metabolomic analysis of zebrafish with/without β-cell ablation and with/without folinic acid treatment indicated β-cell regeneration could be attributed to changes in the pyrimidine, carnitine, and serine pathways. Overall, our results suggest evolutionarily conserved and previously unknown roles for folic acid and one-carbon metabolism in the generation of β-cells.
Document type :
Journal articles
Complete list of metadata

https://hal-univ-lyon1.archives-ouvertes.fr/hal-03666669
Contributor : Brigitte Manship Connect in order to contact the contributor
Submitted on : Thursday, May 12, 2022 - 4:34:55 PM
Last modification on : Friday, May 13, 2022 - 3:36:49 AM

Links full text

Identifiers

Collections

Citation

Christos Karampelias, Habib Rezanejad, Mandy Rosko, Likun Duan, Jing Lu, et al.. Reinforcing one-carbon metabolism via folic acid/Folr1 promotes β-cell differentiation. Nature Communications, Nature Publishing Group, 2021, 12 (1), pp.3362. ⟨10.1038/s41467-021-23673-0⟩. ⟨hal-03666669⟩

Share

Metrics

Record views

0