Skip to Main content Skip to Navigation
Journal articles

Goldfish Response to Chronic Hypoxia: Mitochondrial Respiration, Fuel Preference and Energy Metabolism

Abstract : Hypometabolism is a hallmark strategy of hypoxia tolerance. To identify potential mechanisms of metabolic suppression, we have used the goldfish to quantify the effects of chronically low oxygen (4 weeks; 10% air saturation) on mitochondrial respiration capacity and fuel preference. The responses of key enzymes from glycolysis, beta-oxidation and the tricarboxylic acid (TCA) cycle, and Na+/K+-ATPase were also monitored in various tissues of this champion of hypoxia tolerance. Results show that mitochondrial respiration of individual tissues depends on oxygen availability as well as metabolic fuel oxidized. All the respiration parameters measured in this study (LEAK, OXPHOS, Respiratory Control Ratio, CCCP-uncoupled, and COX) are affected by hypoxia, at least for one of the metabolic fuels. However, no common pattern of changes in respiration states is observed across tissues, except for the general downregulation of COX that may help metabolic suppression. Hypoxia causes the brain to switch from carbohydrates to lipids, with no clear fuel preference in other tissues. It also downregulates brain Na+/K+-ATPase (40%) and causes widespread tissue-specific effects on glycolysis and beta-oxidation. This study shows that hypoxia-acclimated goldfish mainly promote metabolic suppression by adjusting the glycolytic supply of pyruvate, reducing brain Na+/K+-ATPase, and downregulating COX, most likely decreasing mitochondrial density.
Document type :
Journal articles
Complete list of metadata
Contributor : Nathalie Lyvet Connect in order to contact the contributor
Submitted on : Tuesday, April 13, 2021 - 3:35:01 PM
Last modification on : Friday, September 30, 2022 - 11:12:08 AM


Distributed under a Creative Commons Attribution 4.0 International License

Links full text




Elie Farhat, Hang Cheng, Caroline Romestaing, Matthew Pamenter, Jean-Michel Weber. Goldfish Response to Chronic Hypoxia: Mitochondrial Respiration, Fuel Preference and Energy Metabolism. Metabolites, MDPI, 2021, 11 (3), pp.187. ⟨10.3390/metabo11030187⟩. ⟨hal-03197165⟩



Record views