Skip to Main content Skip to Navigation
Journal articles

Plasmid-encoded γ-hexachlorocyclohexane degradation genes and insertion sequences in Sphingobium francense (ex-Sphingomonas paucimobilis Sp+)

Abstract : The lin genes encode the γ-hexachlorocyclohexane (γ-HCH or lindane) catabolic pathway in lindane-degrading strains. The location and stability of these genes have been explored in the lindane-degrading Sphingobium francense strain Sp+, and in two nonlindane-degrading mutants (Sp1- and Sp2-). The lin genes, linA, linB, linE and linX were localized by hybridization on three of the six plasmids of the S. francense strain Sp+ showing dispersal within the genome. The linC gene was detected by PCR, but was not detected by hybridization on any of the plasmids. The hybridization of the linA and linX genes was negative with the two nonlindane-degrading mutants S. francense strains, Sp1- and Sp2-. The dynamic of this genome associated with gene loss and acquisition, and plasmid rearrangement was explored by a search for associated insertion sequences. A new insertion sequence, ISSppa4, belonging to the IS21 family was detected and compared with IS6100 and ISsp1. Insertion sequence localization was explored on different hybridization patterns (plasmid, total genome) with the lindane-degrading Sp+ strain and the two nondegrading derivatives (Sp1-, Sp2-). Insertion sequence movement and plasmid rearrangement could explain the emergence of the nonlindane-degrading mutants.
Document type :
Journal articles
Complete list of metadatas

https://hal-univ-lyon1.archives-ouvertes.fr/hal-02569780
Contributor : Depot 1 Lyon 1 <>
Submitted on : Monday, May 11, 2020 - 3:30:45 PM
Last modification on : Monday, July 13, 2020 - 10:12:01 AM

Links full text

Identifiers

Collections

Citation

Hélène Cérémonie, Hasna Boubakri, Patrick Mavingui, Pascal Simonet, Timothy. M. Vogel. Plasmid-encoded γ-hexachlorocyclohexane degradation genes and insertion sequences in Sphingobium francense (ex-Sphingomonas paucimobilis Sp+). FEMS Microbiology Letters, Wiley-Blackwell, 2006, 257 (2), pp.243-252. ⟨10.1111/j.1574-6968.2006.00188.x⟩. ⟨hal-02569780⟩

Share

Metrics

Record views

12