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Reliability of Pseudomonas aeruginosa semi-automated rep-PCR genotyping in various epidemiological situations

Abstract : Abstract The purpose of this study was to evaluate the possibility of using a semi-automated repetitive DNA sequences-based polymerase chain reaction (rep-PCR) for typing Pseudomonas aeruginosa isolates. rep-PCR profiles obtained by the DiversiLab® system of 84 P. aeruginosa isolates from distinct epidemiological situations were obtained. rep-PCR groupings were in good agreement with the origin of these isolates. Linked rep-PCR profiles were observed for isolates recovered from a same family of cystic fibrosis (CF) patients, for the etiological agents of clustered cases of nosocomial infections, and for some isolates recovered from a same hospital room. rep-PCR and pulsed-field gel electrophoresis SpeI restricted genomic DNA (PFGE-SpeI) profiles were compared. In a few instances, rep-PCR revealed genetic divergences among isolates of a same group of PFGE-SpeI profiles. These divergences could reflect genetic drifts among closely related isolates, as illustrated by those observed between clinical and environmental isolates of a same group of PFGE-SpeI profiles. The interpretation of such differences will require further studies, but the rep-PCR analysis of P. aeruginosa diversity appeared to be an appropriate method to investigate infra-specific genetic relatedness.
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https://hal.archives-ouvertes.fr/hal-00599572
Contributor : Christine Delorme Connect in order to contact the contributor
Submitted on : Friday, June 10, 2011 - 10:44:46 AM
Last modification on : Thursday, September 1, 2022 - 11:15:22 AM

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A. Doléans-Jordheim, B. Cournoyer, E. Bergeron, J. Croizé, H. Salord, et al.. Reliability of Pseudomonas aeruginosa semi-automated rep-PCR genotyping in various epidemiological situations. European Journal of Clinical Microbiology and Infectious Diseases, Springer Verlag, 2009, 28 (9), pp.1105-1111. ⟨10.1007/s10096-009-0755-z⟩. ⟨hal-00599572⟩

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