Skip to Main content Skip to Navigation
Journal articles

The Lid/KDM5 histone demethylase complex activates a critical effector of the oocyte-to- zygote transition

Abstract : Following fertilization of a mature oocyte, the formation of a diploid zygote involves a series of coordinated cellular events that ends with the first embryonic mitosis. In animals, this complex developmental transition is almost entirely controlled by maternal gene products. How such a crucial transcriptional program is established during oogenesis remains poorly understood. Here, we have performed an shRNA-based genetic screen in Drosophila to identify genes required to form a diploid zygote. We found that the Lid/KDM5 histone demethylase and its partner, the Sin3A-HDAC1 deacetylase complex, are necessary for sperm nuclear decompaction and karyogamy. Surprisingly, transcriptomic analyses revealed that these histone modifiers are required for the massive transcriptional activation of deadhead (dhd), which encodes a maternal thioredoxin involved in sperm chromatin remodeling. Unexpectedly, while lid knock-down tends to slightly favor the accumulation of its target, H3K4me3, on the genome, this mark was lost at the dhd locus. We propose that Lid/KDM5 and Sin3A cooperate to establish a local chromatin environment facilitating the unusually high expression of dhd, a key effector of the oocyte-to-zygote transition.
Complete list of metadatas

Cited literature [41 references]  Display  Hide  Download

https://hal-univ-lyon1.archives-ouvertes.fr/hal-02552043
Contributor : Beatrice Horard <>
Submitted on : Thursday, April 23, 2020 - 11:47:52 AM
Last modification on : Saturday, April 25, 2020 - 1:11:07 AM

File

PLoS Genet. 2020 Torres-Campan...
Files produced by the author(s)

Identifiers

Collections

Citation

Daniela Torres-Campana, Shuhei Kimura, Guillermo A. Orsi, Béatrice Horard, Gérard Benoit, et al.. The Lid/KDM5 histone demethylase complex activates a critical effector of the oocyte-to- zygote transition. PLoS Genetics, Public Library of Science, 2020, ⟨10.1371/journal.pgen.1008543⟩. ⟨hal-02552043⟩

Share

Metrics

Record views

57

Files downloads

103