Skip to Main content Skip to Navigation
Journal articles

Comparison of barley succession and take-all disease as environmental factors shaping the rhizobacterial community during take-all decline

Abstract : The root disease take-all, caused by Gaeumannomyces graminis var. tritici, can be managed by monoculture-induced take-all decline (TAD). This natural biocontrol mechanism typically occurs after a take-all outbreak and is believed to arise from an enrichment of antagonistic populations in the rhizosphere. However, it is not known whether these changes are induced by the monoculture or by ecological rhizosphere conditions due to a disease outbreak and subsequent attenuation. This question was addressed by comparing the rhizosphere microflora of barley, either inoculated with the pathogen or noninoculated, in a microcosm experiment in five consecutive vegetation cycles. TAD occurred in soil inoculated with the pathogen but not in noninoculated soil. Bacterial community analysis using terminal restriction fragment length polymorphism of 16S rRNA showed pronounced population shifts in the successive vegetation cycles, but pathogen inoculation had little effect. To elucidate rhizobacterial dynamics during TAD development, a 16S rRNA-based taxonomic microarray was used. Actinobacteria were the prevailing indicators in the first vegetation cycle, whereas the third cycle-affected most severely by take-all-was characterized by Proteobacteria, Bacteroidetes, Chloroflexi, Planctomycetes, and Acidobacteria. Indicator taxa for the last cycle (TAD) belonged exclusively to Proteobacteria, including several genera with known biocontrol traits. Our results suggest that TAD involves monoculture-induced enrichment of plant-beneficial taxa.
Document type :
Journal articles
Complete list of metadatas

https://hal-univ-lyon1.archives-ouvertes.fr/hal-02548061
Contributor : Depot 1 Lyon 1 <>
Submitted on : Monday, April 20, 2020 - 2:31:53 PM
Last modification on : Tuesday, April 21, 2020 - 6:17:21 PM

Links full text

Identifiers

Collections

Citation

K. Schreiner, A. Hagn, Martina Kyselková, Yvan Moënne-Loccoz, G. Welzl, et al.. Comparison of barley succession and take-all disease as environmental factors shaping the rhizobacterial community during take-all decline. Applied and Environmental Microbiology, American Society for Microbiology, 2010, 76 (14), pp.4703-4712. ⟨10.1128/AEM.00481-10⟩. ⟨hal-02548061⟩

Share

Metrics

Record views

17