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Genome wide profiling of Azospirillum lipoferum 4B gene expression during interaction with rice roots

Abstract : Azospirillum-plant cooperation has been mainly studied from an agronomic point of view leading to a wide description of mechanisms implicated in plant growth-promoting effects. However, little is known about genetic determinants implicated in bacterial adaptation to the host plant during the transition from free-living to root-associated lifestyles. This study aims at characterizing global gene expression of Azospirillum lipoferum 4B following a 7-day-old interaction with two cultivars of Oryza sativa L. japonica (cv. Cigalon from which it was originally isolated, and cv. Nipponbare). The analysis was done on a whole genome expression array with RNA samples obtained from planktonic cells, sessile cells and root-adhering cells. Root-associated Azospirillum grow in an active sessile-like state and gene expression is tightly adjusted to the host plant. Adaptation to rice seems to involve genes related to ROS detoxification and multidrug efflux, as well as complex regulatory networks. As revealed by the induction of genes encoding transposases, interaction with root may drive bacterial genome rearrangements. Several genes related to ABC transporter and ROS detoxification display cultivar-specific expression profiles, suggesting host specific adaptation and raising the question of A. lipoferum 4B/rice cv. Cigalon co-adaptation.This article is protected by copyright. All rights reserved.
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https://hal-univ-lyon1.archives-ouvertes.fr/hal-02522211
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Submitted on : Friday, March 27, 2020 - 5:58:35 PM
Last modification on : Tuesday, March 31, 2020 - 2:58:08 PM

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B. Drogue, H. Sanguin, S. Borland, Claire Prigent-Combaret, Florence Wisniewski-Dyé. Genome wide profiling of Azospirillum lipoferum 4B gene expression during interaction with rice roots. FEMS Microbiology Ecology, Wiley-Blackwell, 2013, ⟨10.1111/1574-6941.12244⟩. ⟨hal-02522211⟩

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