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Cell culturability of Pseudomonas protegens CHA0 depends on soil pH

Abstract : Pseudomonas inoculants may lose colony-forming ability in soil, but soil properties involved are poorly documented. Here, we tested the hypothesis that soil acidity could reduce persistence and cell culturability of Pseudomonas protegens CHA0. At one week in vitro, strain CHA0 was found as culturable cells at pH 7, whereas most cells at pH 4 and all cells at pH 3 were non-cultured. In 21 natural soils of contrasted pH, cell culturability loss of P. protegens CHA0 took place in all six very acidic soils (pH \\textless 5.0) and in three of five acidic soils (5.0 \\textless pH \\textless 6.5), whereas it was negligible in the neutral and alkaline soils at two weeks and two months. No correlation was found between total cell counts of P. protegens CHA0 and soil composition data, whereas colony counts of the strain correlated with soil pH. Maintenance of cell culturability in soils coincided with a reduction of inoculant cell size. Some of the non-cultured CHA0 cells were nutrient-responsive in Kogure's viability test, both in vitro and in soil. Thus, this shows for the first time that the sole intrinsic soil composition factor triggering cell culturability loss in P. protegens CHA0 is soil acidity.
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https://hal-univ-lyon1.archives-ouvertes.fr/hal-02522199
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Submitted on : Friday, March 27, 2020 - 5:57:59 PM
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F. Mascher, C. Hase, Marie.-Lara Bouffaud, G. Défago, Yvan Moënne-Loccoz. Cell culturability of Pseudomonas protegens CHA0 depends on soil pH. FEMS Microbiology Ecology, Wiley-Blackwell, 2013, 87 (2), pp.441-450. ⟨10.1111/1574-6941.12234⟩. ⟨hal-02522199⟩

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