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Direct Identification of Staphylococcus aureus and Determination of Methicillin Susceptibility From Positive Blood-Culture Bottles in a Bact/ALERT System Using Binax Now \textitS. aureus and PBP2a Tests

Abstract : Staphylococcus aureus bacteremia is associated with high mortality and morbidity, requiring prompt and appropriate antimicrobial treatment. Therefore, it is important to detect methicillin-resistant S. aureus (MRSA) rapidly from blood cultures. Two immunochromatographic tests, BinaxNow S. aureus and BinaxNow PBP2a, were directly applied to 79 Bact/Alert bottles that were positive for Gram positive cocci in cluster aggregations. Sensitivity and specificity for the identification of S. aureus and determination of methicillin resistance were 94% and 87%, and 100% and 100%, respectively, with less than 30 min of performance time. These tests are efficient and rapid; these tests are valuable alternatives to more sophisticated and expensive methods used in the diagnosis of MRSA bacteremia.
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https://hal-univ-lyon1.archives-ouvertes.fr/hal-02487306
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Submitted on : Friday, February 21, 2020 - 2:56:57 PM
Last modification on : Wednesday, May 27, 2020 - 10:19:29 PM

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S. Heraud, A.-M. Freydiere, Anne Doleans-Jordheim, M. Bes, A. Tristan, et al.. Direct Identification of Staphylococcus aureus and Determination of Methicillin Susceptibility From Positive Blood-Culture Bottles in a Bact/ALERT System Using Binax Now \textitS. aureus and PBP2a Tests. Annals of Laboratory Medicine, Korean Society for Laboratory Medicine, 2015, 35 (4), pp.454. ⟨10.3343/alm.2015.35.4.454⟩. ⟨hal-02487306⟩

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