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Journal articles
Omics of the early molecular dialogue between Frankia alni and Alnus glutinosa and the cellulase synton
Abstract : The early Frankia-Alnus symbiotic molecular exchanges were analyzed in detail by protein and RNA omics. For this, Frankia cells were placed in the presence of Alnus roots but separated by a dialysis membrane for 64 h. The bacterial cells were then harvested and analyzed by high-throughput proteomics and transcriptomics (RNA-seq). The most upregulated gene clusters were found to be the potassium transporter operon kdp and an ABC transporter operon of uncharacterized function. The most upregulated proteins were found to be acyl dehydrogenases and the potassium transporter Kdp. These suggest a preadaptation to the impending stresses linked to the penetration into isotonic host tissues and a possible rearrangement of the membrane. Another cluster among the 60 most upregulated ones that comprised two cellulases and a cellulose synthase was conserved among the Frankia and other actinobacteria such as Streptomyces. Cellulase activity was detected on CMC all along the length of the root but not away from it. Frankia alni ACN14a was found to be unable to respire or grow on glucose as sole carbon source. The cellulose synthase was found active at the tip of hyphae in response to Alnus root exudates, resulting in a calcofluor stained tip.
https://hal-univ-lyon1.archives-ouvertes.fr/hal-02473974
Contributor : Depot 1 Lyon 1 Connect in order to contact the contributor
Submitted on : Tuesday, February 11, 2020 - 10:10:27 AM
Last modification on : Wednesday, November 3, 2021 - 7:37:51 AM
Contributor : Depot 1 Lyon 1 Connect in order to contact the contributor
Submitted on : Tuesday, February 11, 2020 - 10:10:27 AM
Last modification on : Wednesday, November 3, 2021 - 7:37:51 AM