Comparison of kava ( Piper methysticum Forst.) varieties by UV absorbance of acetonic extracts and high-performance thin-layer chromatography

Abstract : The development of quality standards to regulate the trade of kava (Piper methysticum) in the Pacific necessitates new analytical techniques for routine control of high-quality raw material. In the present study, varieties and parts of the plant were compared for their kavalactone and flavokavin (FK) profiles. Three hundred and eight samples corresponding to 25 noble, 25 two-days and five wichmannii varieties were collected from 203 different plants. Commercial samples were also analysed to test the technique. Overall, 353 samples were prepared (221 roots, 114 stumps, nine basal stems and nine stem peelings). Their acetonic extracts were measured for UV absorbance and analysed by HP-TLC. Two ratios were computed after scanning the plates at 245nm (K/KL=kavain/total kavalactones) and 366nm (FK/KL=flavokavins/kavalactones). The results indicate that noble varieties suitable for daily consumption of kava are characterised by high K/KL and low FK/KL. At 400nm, the mean UV absorbance of acetonic extracts from noble, two-days and wichmannii varieties are 0.69, 0.94 and 1.04, respectively. The significant (+0.757**) correlation between the total FK content and the extracts absorbance at 400nm indicates that a simple colorimeter can be used to detect poor-quality raw material.
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Submitted on : Tuesday, February 4, 2020 - 11:33:24 AM
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Vincent Lebot, Laurent Legendre. Comparison of kava ( Piper methysticum Forst.) varieties by UV absorbance of acetonic extracts and high-performance thin-layer chromatography. Journal of Food Composition and Analysis, Elsevier, 2016, 48, pp.25-33. ⟨10.1016/j.jfca.2016.01.009⟩. ⟨hal-02466016⟩

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