In vitro and in vivo anti-melanoma effects of Daphne gnidium aqueous extract via activation of the immune system

Mounira Krifa Kamel Ghedira Leila Chekir-Ghedira Fadwa Chaabane Nadia Mustapha Imen Mokdad-Bzeouich Aicha Sassi Soumaya Kilani-Jaziri Marie-Geneviève Dijoux Franca 1 Serge Michalet 1 Mayssa Fathallah
1 LEM - Laboratoire d'Ecologie Microbienne - UMR 5557
Abstract : The purpose of this study was to assess the antitumor and immunomodulatory effects of the aqueous extract from Daphne gnidium in mice-bearing melanoma tumor. Balb/C mice were subcutaneously implanted with B16-F10 cells and treated intraperitoneally with the aqueous extract at 200 mg/Kg b.w for 21 days. After euthanization on day 22, the tumors were weighed; lymphocyte proliferation, cytotoxic T lymphocyte (CTL), and natural killer (NK) cell activities were evaluated using the MTT assay. Macrophage phagocytosis was studied by measuring the lysosomal activity. In addition to its potential to inhibit the growth of the transplantable tumor, the aqueous extract remarkably induced splenocyte proliferation and both NK and CTL activities in tumor-bearing mice. The aqueous extract was also seen to have promoted lysosomal activity of host macrophages.
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Submitted on : Tuesday, February 4, 2020 - 11:33:12 AM
Last modification on : Monday, February 10, 2020 - 4:36:45 PM

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Mounira Krifa, Kamel Ghedira, Leila Chekir-Ghedira, Fadwa Chaabane, Nadia Mustapha, et al.. In vitro and in vivo anti-melanoma effects of Daphne gnidium aqueous extract via activation of the immune system. Tumor Biology, 2016, 37 (5), pp.6511-6517. ⟨10.1007/s13277-015-4492-x⟩. ⟨hal-02466013⟩

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