Skip to Main content Skip to Navigation
Journal articles

Comparative genomic analysis identifies small open reading frames (sORFs) with peptide-encoding features in avian 16S rDNA

Abstract : The mitochondrial genome (mt-DNA) functional repertoire has recently been enriched in mammals by the identification of functional small open reading frames (sORFs) embedded in ribosomal DNAs. Through com- parative genomic analyses the presence of putatively functional sORFs was investigated in birds. Alignment of available avian mt-DNA sequences revealed highly conserved regions containing four putative sORFs that pre- sented low insertion/deletion polymorphism rate (< 0.1%) and preserved in frame start/stop codons in > 80% of species. Detected sORFs included avian homologs of human Humanin and Short-Humanin-Like-Peptide 6 and two new sORFs not yet described in mammals. The amino-acid sequences of the four putative encoded peptides were strongly conserved among birds, with amino-acid p-distances (5.6 to 25.4%) similar to those calculated for typical avian mt-DNA-encoded proteins (14.8%). Conservation resulted from either drastic conservation of the nucleotide sequence or negative selection pressure. These data extend to birds the possibility that mitochondrial rDNA may encode small bioactive peptides.
Document type :
Journal articles
Complete list of metadatas

https://hal-univ-lyon1.archives-ouvertes.fr/hal-02345550
Contributor : Nathalie Lyvet <>
Submitted on : Monday, November 4, 2019 - 3:32:24 PM
Last modification on : Thursday, May 14, 2020 - 2:30:22 PM

Identifiers

Collections

Citation

Mathieu Mortz, Cyril Degletagne, Caroline Romestaing, Claude Duchamp. Comparative genomic analysis identifies small open reading frames (sORFs) with peptide-encoding features in avian 16S rDNA. Genomics, Elsevier, 2020, 112 (2), pp.1120-1127. ⟨10.1016/j.ygeno.2019.06.026⟩. ⟨hal-02345550⟩

Share

Metrics

Record views

86