Service interruption on Monday 11 July from 12:30 to 13:00: all the sites of the CCSD (HAL, EpiSciences, SciencesConf, AureHAL) will be inaccessible (network hardware connection).
Skip to Main content Skip to Navigation
Journal articles

Water Dynamics in Shewanella oneidensis at Ambient and High Pressure using Quasi-Elastic Neutron Scattering

Abstract : Quasielastic neutron scattering (QENS) is an ideal technique for studying water transport and relaxation dynamics at pico-to nanosecond timescales and at length scales relevant to cellular dimensions. Studies of high pressure dynamic effects in live organisms are needed to understand Earth's deep biosphere and biotechnology applications. Here we applied QENS to study water transport in Shewanella oneidensis at ambient (0.1 MPa) and high (200 MPa) pressure using H/D isotopic contrast experiments for normal and perdeuterated bacteria and buffer solutions to distinguish intracellular and transmembrane processes. The results indicate that intracellular water dynamics are comparable with bulk diffusion rates in aqueous fluids at ambient conditions but a significant reduction occurs in high pressure mobility. We interpret this as due to enhanced interactions with macromolecules in the nanoconfined environment. Overall diffusion rates across the cell envelope also occur at similar rates but unexpected narrowing of the QENS signal appears between momentum transfer values Q = 0.7-1.1 angstrom(-1) corresponding to real space dimensions of 6-9 angstrom. The relaxation time increase can be explained by correlated dynamics of molecules passing through Aquaporin water transport complexes located within the inner or outer membrane structures.
Document type :
Journal articles
Complete list of metadata
Contributor : Depot 2 Lyon 1 Connect in order to contact the contributor
Submitted on : Thursday, December 31, 2020 - 7:47:38 AM
Last modification on : Saturday, January 22, 2022 - 6:48:02 PM
Long-term archiving on: : Thursday, April 1, 2021 - 6:07:21 PM


Publisher files allowed on an open archive


Distributed under a Creative Commons Attribution - NoDerivatives 4.0 International License




Fabrizia Foglia, Rachael Hazael, Giovanna G. Simeoni, Marie-Sousai Appavou, Martine Moulin, et al.. Water Dynamics in Shewanella oneidensis at Ambient and High Pressure using Quasi-Elastic Neutron Scattering. Scientific Reports, Nature Publishing Group, 2016, 6, ⟨10.1038/srep18862⟩. ⟨hal-02331143⟩



Record views


Files downloads