Erythroid differentiation displays a peak of energy consumption concomitant with glycolytic metabolism rearrangements

Angélique Richard 1 Elodie Vallin 1 Caroline Romestaing 2 Damien Roussel 2 Olivier Gandrillon 1, 3 Sandrine Gonin-Giraud 1
1 LBMC UMR 5239 - Laboratoire de biologie et modélisation de la cellule
2 LEHNA - Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés
3 DRACULA - Multi-scale modelling of cell dynamics : application to hematopoiesis
CGPhiMC - Centre de génétique et de physiologie moléculaire et cellulaire, Inria Grenoble - Rhône-Alpes, ICJ - Institut Camille Jordan [Villeurbanne]
Abstract : Our previous single-cell based gene expression analysis pointed out significant variations of LDHA level during erythroid differentiation. Deeper investigations highlighted that a metabolic switch occurred along differentiation of erythroid cells. More precisely we showed that self-renewing progenitors relied mostly upon lactate-productive glycolysis, and required LDHA activity, whereas differentiating cells, mainly involved mitochondrial oxidative phosphorylation (OXPHOS). These metabolic rearrangements were coming along with a particular temporary event, occurring within the first 24h of erythroid differentiation. The activity of glycolytic metabolism and OXPHOS rose jointly with oxgene consumption dedicated to ATP production at 12-24h of the differentiation process before lactate-productive glycolysis sharply fall down and energy needs decline. Finally, we demonstrated that the metabolic switch mediated through LDHA drop and OXPHOS upkeep might be necessary for erythroid differentiation. We also discuss the possibility that metabolism, gene expression and epigenetics could act together in a circular manner as a driving force for differentiation.
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https://hal-univ-lyon1.archives-ouvertes.fr/hal-02318812
Contributor : Nathalie Lyvet <>
Submitted on : Thursday, October 17, 2019 - 2:33:50 PM
Last modification on : Friday, February 28, 2020 - 3:01:10 PM

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Angélique Richard, Elodie Vallin, Caroline Romestaing, Damien Roussel, Olivier Gandrillon, et al.. Erythroid differentiation displays a peak of energy consumption concomitant with glycolytic metabolism rearrangements. PLoS ONE, Public Library of Science, 2019, 14 (9), pp.e0221472. ⟨10.1371/journal.pone.0221472⟩. ⟨hal-02318812⟩

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