3D imaging of theranostic nanoparticles in mice organs by means of x-ray phase contrast tomography

Elena Longo 1 Alberto Bravin 2 Francesco Brun 3 Inna Bukreeva 3 Alessia Cedola 3 Ombeline de la Rochefoucauld 4 Michela Fratini 3, 5 Xavier Le Guével 6 Lorenzo Massimi 3 Lucie Sancey 6 Olivier Tillement 7 Philippe Zeitoun 1, *
* Corresponding author
1 LOA - Laboratoire d'optique appliquée
2 ESRF - European Synchrotron Radiation Facility
3 CNR NANO - Institute of Nanotechnology [Modena, MO, Italy]
4 Imagine Optic
5 IRCCS - Foundation Saint Lucia [Roma, Italy]
6 IAB - Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble)
7 ILM - Institut Lumière Matière [Villeurbanne]
Abstract : Theranostics is an innovative research field that aims to develop high target specificity cancer treatments by administering small metal-based nanoparticles (NPs). This new generation of compounds exhibits diagnostic and therapeutic properties due to the high atomic number of their metal component. In the framework of a combined research program on low dose X-ray imaging and theranostic NPs, X-ray Phase Contrast Tomography (XPCT) was performed at ESRF using a 3 μm pixel optical system on two samples: a mouse brain bearing melanoma metastases injected with gadolinium NPs and, a mouse liver injected with gold NPs. XPCT is a non-destructive technique suitable to achieve the 3D reconstruction of a specimen and, widely used at micro-scale to detect abnormalities of the vessels, which are associated to the tumor growth or to the development of neurodegenerative diseases. Moreover, XPCT represents a promising and complementary tool to study the biodistribution of theranostic NPs in biological materials, thanks to the strong contrast with respect to soft tissues that metal-based NPs provide in radiological images. This work is relied on an original imaging approach based on the evaluation of the contrast differences between the images acquired below and above K-edge energies, as a proof of the certain localization of NPs. We will present different methods aiming to enhance the localization of NPs and a 3D map of their distribution in large volume of tissues.
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Conference papers
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https://hal-univ-lyon1.archives-ouvertes.fr/hal-02316354
Contributor : Marie-Gabrielle Chautard <>
Submitted on : Tuesday, October 15, 2019 - 11:31:44 AM
Last modification on : Wednesday, November 20, 2019 - 2:29:46 AM

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UNIV-LYON1 | X-DEP-PHYS | X-LOA | CNRS | ENSTA_LOA | X | ENSTA | UNIV-PARIS-SACLAY

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Elena Longo, Alberto Bravin, Francesco Brun, Inna Bukreeva, Alessia Cedola, et al.. 3D imaging of theranostic nanoparticles in mice organs by means of x-ray phase contrast tomography. Physics of Medical Imaging, Feb 2018, Houston, France. pp.167, ⟨10.1117/12.2293090⟩. ⟨hal-02316354⟩

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