Analysis of collagen expression during chondrogenic induction of human bone marrow mesenchymal stem cells

Emeline Perrier-Groult 1 Marie-Claire Ronzière 1 Reine Bareille 2 Astrid Pinzano 3 Frédéric Mallein-Gerin 1 Anne-Marie Freyria 1
1 IBCP - Institut de biologie et chimie des protéines [Lyon]
2 Université de Bordeaux Ségalen [Bordeaux 2]
3 PPIA - Physiopathologie, Pharmacologie et Ingénierie articulaires
Abstract : Adult mesenchymal stem cells (MSCs) are currently being investigated as an alternative to chondrocytes for repairing cartilage defects. As several collagen types participate in the formation of cartilage-specific extracellular matrix, we have investigated their gene expression levels during MSC chondrogenic induction. Bone marrow MSCs were cultured in pellet in the presence of BMP-2 and TGF-β3 for 24 days. After addition of FGF-2, at the fourth passage during MSC expansion, there was an enhancing effect on specific cartilage gene expression when compared to that without FGF-2 at day 12 in pellet culture. A switch in expression from the pre-chondrogenic type IIA form to the cartilage-specific type IIB form of the collagen type II gene was observed at day 24. A short-term addition of FGF-2 followed by a treatment with BMP-2/TGF-β3 appears sufficient to accelerate chondrogenesis with a particular effect on the main cartilage collagens.
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https://hal-univ-lyon1.archives-ouvertes.fr/hal-02200278
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Submitted on : Wednesday, July 31, 2019 - 10:20:03 AM
Last modification on : Tuesday, November 19, 2019 - 2:37:36 AM

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Emeline Perrier-Groult, Marie-Claire Ronzière, Reine Bareille, Astrid Pinzano, Frédéric Mallein-Gerin, et al.. Analysis of collagen expression during chondrogenic induction of human bone marrow mesenchymal stem cells. Biotechnology Letters, Springer Verlag, 2011, 33 (10), pp.2091-2101. ⟨10.1007/s10529-011-0653-1⟩. ⟨hal-02200278⟩

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