 |
Journal articles
Analysis of collagen expression during chondrogenic induction of human bone marrow mesenchymal stem cells
Abstract : Adult mesenchymal stem cells (MSCs) are currently being investigated as an alternative to chondrocytes for repairing cartilage defects. As several collagen types participate in the formation of cartilage-specific extracellular matrix, we have investigated their gene expression levels during MSC chondrogenic induction. Bone marrow MSCs were cultured in pellet in the presence of BMP-2 and TGF-β3 for 24 days. After addition of FGF-2, at the fourth passage during MSC expansion, there was an enhancing effect on specific cartilage gene expression when compared to that without FGF-2 at day 12 in pellet culture. A switch in expression from the pre-chondrogenic type IIA form to the cartilage-specific type IIB form of the collagen type II gene was observed at day 24. A short-term addition of FGF-2 followed by a treatment with BMP-2/TGF-β3 appears sufficient to accelerate chondrogenesis with a particular effect on the main cartilage collagens.
https://hal-univ-lyon1.archives-ouvertes.fr/hal-02200278
Contributor : Emeline Groult <>
Submitted on : Wednesday, July 31, 2019 - 10:20:03 AM
Last modification on : Wednesday, November 25, 2020 - 9:52:01 PM
Contributor : Emeline Groult <>
Submitted on : Wednesday, July 31, 2019 - 10:20:03 AM
Last modification on : Wednesday, November 25, 2020 - 9:52:01 PM
Links full text
