Characterization of a novel canine T-cell line established from a spontaneously occurring aggressive T-cell lymphoma with large granular cell morphology

Abstract : Dogs with lymphoma are established as good model for human non-Hodgkin lymphoma studies. Canine cell lines derived from lymphomas may be valuable tools for testing new therapeutic drugs. In this context, we established a canine T-cell line, PER-VAS, from a primary aggressive T-cell lymphoma with large granular morphology. Flow cytometric analysis revealed a stable immunophenotype: PER-VAS cells were positively labelled for CD5, CD45, MHC II and TLR3, and were negative for CD3, CD4 and CD8 expression. Although unstable along the culture process, IL-17 and MMP12 proteins were detectable as late as at passages 280 and 325i.e. respectively 24 and 29 months post isolation. At passage 325, PER-VAS cells maintained the expression of IL-17, CD3, CD56, IFNgamma and TNFalpha mRNAs as shown by RT-PCR analysis. Stable rearrangement of the TCRgamma gene has been evidenced by PCR. PER-VAS cells have a high proliferation index with a doubling time of 16.5h and were tumorigenic in Nude mice. Compared to the canine cell lines already reported, PER-VAS cells display an original expression pattern, close to NKT cells, which makes them valuable tools for in vitro comparative research on lymphomas.
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Contributor : Marie-Gabrielle Chautard <>
Submitted on : Wednesday, July 24, 2019 - 10:55:40 AM
Last modification on : Tuesday, September 24, 2019 - 4:23:47 PM

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C. Bonnefont-Rebeix, C. Fournel-Fleury, F. Ponce, S. Belluco, D. Watrelot, et al.. Characterization of a novel canine T-cell line established from a spontaneously occurring aggressive T-cell lymphoma with large granular cell morphology. Immunobiology, Elsevier, 2016, 221 (1), pp.12-22. ⟨10.1016/j.imbio.2015.08.007⟩. ⟨hal-02192918⟩

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