Porcine endogenous retrovirus-A/C: biochemical properties of its integrase and susceptibility to raltegravir

Abstract : Porcine endogenous retroviruses (PERVs) are present in the genomes of pig cells. The PERV-A/C recombinant virus can infect human cells and is a major risk of zoonotic disease in the case of xenotransplantation of pig organs to humans. Raltegravir (RAL) is a viral integrase (IN) inhibitor used in highly active antiretroviral treatment. In the present study, we explored the potential use of RAL against PERV-A/C. We report (i) a three-dimensional model of the PERV-A/C intasome complexed with RAL, (ii) the sensitivity of PERV-A/C IN to RAL in vitro and (iii) the sensitivity of a PERV-A/C-IRES-GFP recombinant virus to RAL in cellulo. We demonstrated that RAL is a potent inhibitor against PERV-A/C IN and PERV-A/C replication with IC50s in the nanomolar range. To date, the use of retroviral inhibitors remains the only way to control the risk of zoonotic PERV infection during pig-to-human xenotransplantation.
Document type :
Journal articles
Complete list of metadatas

https://hal-univ-lyon1.archives-ouvertes.fr/hal-02192915
Contributor : Marie-Gabrielle Chautard <>
Submitted on : Wednesday, July 24, 2019 - 10:55:33 AM
Last modification on : Thursday, July 25, 2019 - 1:28:29 AM

Links full text

Identifiers

Collections

Citation

A. Demange, Halima Yajjou-Hamalian, Kathy Gallay, Catherine Luengo, V. Beven, et al.. Porcine endogenous retrovirus-A/C: biochemical properties of its integrase and susceptibility to raltegravir. Journal of General Virology, 2015, 96 (10), pp.3124-30. ⟨10.1099/jgv.0.000236⟩. ⟨hal-02192915⟩

Share

Metrics

Record views

7