Skip to Main content Skip to Navigation
Journal articles

Activation of the mTOR/Akt pathway in thymic epithelial cells derived from thymomas

Abstract : The pathogenesis of thymic epithelial tumors remains poorly elucidated. The PIK3/Akt/mTOR pathway plays a key role in various cancers; interestingly, several phase I/II studies have reported a positive effect of mTOR inhibitors in disease control in thymoma patients. A major limit for deciphering cellular and molecular events leading to the transformation of thymic epithelial cells or for testing drug candidates is the lack of reliable in vitro cell system. We analyzed protein expression and activation of key players of the Akt/ mTOR pathway namely Akt, mTOR, and P70S6K in eleven A, B and AB thymomas as well as in normal thymuses. While only Akt and phospho-Akt were expressed in normal thymuses, both Akt and mTOR were activated in thymomas. Phospho-P70S6K was expressed in all thymic tumors whatever their subtypes, and absent in normal thymus. Interestingly, we report the activation of Akt, mTOR and P70S6 proteins in primary thymic epithelial cells maintained for short period of time after their derivation from seven AB and B thymomas. Finally, we showed that rapamycin (100 nM) significantly reduced proliferation of thymoma- derived epithelial cells without inducing cell death. Our results suggest that the activation of the Akt/ mTOR pathway might participate to the cell proliferation associated with tumor growth. Ultimately, our data enhance the potential role of thymic epithelial cells derived from tissue specimens for in vitro exploration of molecular abnormalities in rare thymic tumors.
Document type :
Journal articles
Complete list of metadatas

Cited literature [40 references]  Display  Hide  Download

https://hal-univ-lyon1.archives-ouvertes.fr/hal-02191888
Contributor : Marie-Gabrielle Chautard <>
Submitted on : Tuesday, May 26, 2020 - 11:43:13 PM
Last modification on : Wednesday, October 14, 2020 - 4:18:51 AM

File

2019_Maury_Plos_one_1.pdf
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Jean-Michel Maury, Claire Merveilleux Du Vignaux, Gabrielle Drevet, V. Zarza, L. Chalabreysse, et al.. Activation of the mTOR/Akt pathway in thymic epithelial cells derived from thymomas. PLoSOne, 2019, 14 (3), pp.e019765. ⟨10.1371/journal.pone.0197655⟩. ⟨hal-02191888⟩

Share

Metrics

Record views

108

Files downloads

87