Determination of Root Exudate Concentration in the Rhizosphere Using 13C Labeling

Abstract : One of the most remarkable metabolic features of plant roots is their ability to secrete a wide range of compounds into the rhizosphere, defined as the volume of soil around living roots. Around 5%- 21% of total photosynthetically fixed carbon is transferred into the rhizosphere through root exudates. Until recently, studies on the quantity and quality of root exudates were conducted mostly under axenic or monoxenic in vitro conditions. Today, in situ assays are required to provide a better understanding of root exudates dynamics and role in plant-microbe interactions. By incubating plants with 13CO2 in situ for one week and quantifying 13C enrichment from the root-adhering soil using mass spectrometry, we were able to determine root exudate levels. Indeed, labeled substrate 13CO2 is converted into organic carbon via plant photosynthesis and transferred into the soil through root exudation. We assume that all 13C increases above natural abundance are mainly derived from exudates produced by 13C-labeled plants.
Document type :
Journal articles
Complete list of metadatas

https://hal-univ-lyon1.archives-ouvertes.fr/hal-02148480
Contributor : Nathalie Lyvet <>
Submitted on : Wednesday, June 5, 2019 - 3:17:26 PM
Last modification on : Thursday, June 6, 2019 - 1:23:10 AM

Identifiers

Collections

Citation

Laurent Simon, Feth El Zahar Haichar. Determination of Root Exudate Concentration in the Rhizosphere Using 13C Labeling. Bio-protocol , Bio-protocol LCC, 2019, 9 (9), pp.1-7. ⟨10.21769/BioProtoc.3228⟩. ⟨hal-02148480⟩

Share

Metrics

Record views

13