Toxoplasma gondii: Identification and immune response against a group of proteins involved in cellular invasion

Samira Azzouz 1 Mimoun Maache 2 Antonio Osuna 2 Philippe Lawton 1 Anne Françoise Pétavy 3
1 UCBL - Université Claude Bernard Lyon 1
2 UGR - Universidad de Granada
3 ISPB - Institut des Sciences Pharmaceutiques et Biologiques
Abstract : Toxoplasma gondii is an ubiquitous intracellular parasite, causative agent of toxoplasmosis, and a worldwide zoonosis for which an effective vaccine is needed. A group of proteins secreted by tachyzoites during host-cell invasion was isolated from the interaction medium. It induced the permeability of the cells as assessed by alpha-sarcin and consequently facilitated the entry of the parasite into the cells. SDS-PAGE of the purified proteins showed a pattern of four proteins of 67, 42, 32 and 27 kDa. MRC-5 cells incubated with the total protein and the different electroeluted bands endured a high cellular death in presence of alpha-sarcin. BALb/C mice immunized with the group of proteins had a mixed Th1/Th2 response and were protected upon challenge with the parasites.
Keywords : Permeability Vaccine Cellular invasion Toxoplasma gondii
Document type :
Journal articles
Complete list of metadatas
https://hal-univ-lyon1.archives-ouvertes.fr/hal-02111025
Contributor : Philippe Lawton <>
Submitted on : Friday, April 26, 2019 - 2:27:03 PM
Last modification on : Friday, April 26, 2019 - 3:57:41 PM

File

19-Azzouz 12 modifié.pdf
Files produced by the author(s)

Identifiers

Collections

UNIV-LYON1

Citation

Samira Azzouz, Mimoun Maache, Antonio Osuna, Philippe Lawton, Anne Françoise Pétavy. Toxoplasma gondii: Identification and immune response against a group of proteins involved in cellular invasion. Experimental Parasitology, Elsevier, 2012, 130 (1), pp.63-68. ⟨10.1016/j.exppara.2011.09.012⟩. ⟨hal-02111025⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

9

Files downloads

24

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD