Toxoplasma gondii: Identification and immune response against a group of proteins involved in cellular invasion

Abstract : Toxoplasma gondii is an ubiquitous intracellular parasite, causative agent of toxoplasmosis, and a worldwide zoonosis for which an effective vaccine is needed. A group of proteins secreted by tachyzoites during host-cell invasion was isolated from the interaction medium. It induced the permeability of the cells as assessed by alpha-sarcin and consequently facilitated the entry of the parasite into the cells. SDS-PAGE of the purified proteins showed a pattern of four proteins of 67, 42, 32 and 27 kDa. MRC-5 cells incubated with the total protein and the different electroeluted bands endured a high cellular death in presence of alpha-sarcin. BALb/C mice immunized with the group of proteins had a mixed Th1/Th2 response and were protected upon challenge with the parasites.
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Samira Azzouz, Mimoun Maache, Antonio Osuna, Philippe Lawton, Anne Françoise Pétavy. Toxoplasma gondii: Identification and immune response against a group of proteins involved in cellular invasion. Experimental Parasitology, Elsevier, 2012, 130 (1), pp.63-68. ⟨10.1016/j.exppara.2011.09.012⟩. ⟨hal-02111025⟩

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