Overexpression, purification and characterization of a hexahistidine-tagged recombinant extended nucleotide-binding domain 1 (NBD1) of the Cryptosporidium parvum CpABC3 for rational drug design

Philippe Lawton 1, 2 Michel Pélandakis 3 Anne-Françoise Pétavy 2 Nadia Walchshofer 4
1 Laboratoire de Parasitologie
2 UCBL - Université Claude Bernard Lyon 1
3 A&I - Amibes et interactions
MAP - Microbiologie, adaptation et pathogénie
4 B2MC - Molécules bioactives et chimie médicinale
Abstract : Its natural resistance to antiprotozoal chemotherapy characterizes the intestinal protozoan parasite Cryptosporidium parvum and the P-glycoprotein-related multidrug resistance proteins such as CpABC3 could be involved. In order to design and study specific inhibitors of the CpABC3 nucleotide-binding domain, a hexahistidine-tagged recombinant protein encompassing the N-terminal cytosolic NBD1 domain was over-expressed in E. coli and purified. The 45 kDa H6-NBD1 displayed intrinsic fluorescent properties consistent with the presence of two Trp residues in a hydrophobic environment. The binding of ATP and the fluorescent analogue TNP-ATP produced a dose-dependent quenching as well as progesterone and the flavone quercetin. The extrinsic fluorescence of TNP-ATP was enhanced upon binding to H6-NBD1, which was only partially displaced by the natural substrate ATP. The recombinant protein hydrolyzed ATP (K m = 145.4 ± 18.2 M), but ADP (K m = 4.3 ± 0.6 mM) and AMP (K m = 5.4 ± 1.5 M) were also substrates. TNP-ATP is a competitive inhibitor of the catalytic activity (K i = 36.6 ± 4.5 M), but quercetin and progesterone were not inhibitors, evidencing different binding sites. The recombinant C. parvum H6-NBD1 should be a valuable tool for rational drug design and will allow the discrimination between specific inhibitors of the catalytic site and molecules binding to other sites.
Keywords : CpABC3 NBD1 Parasite Cryptosporidium parvum Nucleotide-binding domain
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Journal articles
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Submitted on : Friday, April 26, 2019 - 3:54:17 PM
Last modification on : Tuesday, November 19, 2019 - 2:39:22 AM

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UNIV-LYON1 | CNRS | BIOENVIS | INSA-LYON | B2MC | UMR5240 | INSA-GROUPE | UDL

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Philippe Lawton, Michel Pélandakis, Anne-Françoise Pétavy, Nadia Walchshofer. Overexpression, purification and characterization of a hexahistidine-tagged recombinant extended nucleotide-binding domain 1 (NBD1) of the Cryptosporidium parvum CpABC3 for rational drug design. Molecular and Biochemical Parasitology, Elsevier, 2007, 152 (1), pp.101-107. ⟨10.1016/j.molbiopara.2006.12.004⟩. ⟨hal-02111014⟩

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