Effects of purine nucleosides on the in vitro growth of Cryptosporidium parvum

Philippe Lawton (1, 1) , Carine Hejl (2) , Roselyne Mancassola (3) , Muriel Naciri (3) , Anne-Françoise Pétavy (2)

1 UCBL - Université Claude Bernard Lyon 1
2 UCBL ISPB - Université Claude Bernard Lyon 1 - Institut des Sciences Pharmaceutiques et Biologiques
3 PAP - Station de Pathologie aviaire et parasitologie [Nouzilly]

The effect of purine nucleosides on the in vitro growth of Cryptosporidium parvum was studied. Culturing the parasite in THP-1 cells for 72 h in growth medium supplemented with adenosine or inosine improved the parasite yields especially in the first 48 h. Similar results were obtained with parasites cultured in Madin^Darby bovine kidney cells and incubated for 24 h with inosine. The addition of inosine to 72-h cultures enhanced the growth of C. parvum in THP-1 cells, especially the trophic stages, whereas the analogue formycin B was toxic to the parasites and induced a marked decrease in the gamont stages. The monitoring of the added purine nucleosides by high performance liquid chromatography showed that at 37 ‡C in the presence of THP-1 cells, a rapid uptake of inosine occurred with hypoxanthine being the main purine present after 2 h in the medium.

Mots clés: In vitro culture; THP-1 cell; Inosine; Cryptosporidium parvum; Adenosine

Domaines: Parasitologie

Philippe Lawton, Carine Hejl, Roselyne Mancassola, Muriel Naciri, Anne-Françoise Pétavy. Effects of purine nucleosides on the in vitro growth of Cryptosporidium parvum. FEMS Microbiology Letters, 2003, 226 (1), pp.39-43. ⟨10.1016/S0378-1097(03)00555-X⟩. ⟨hal-02110999⟩