Echinococcus multilocularis Alkaline Phosphatase as a Marker for Metacestode Damage Induced by In Vitro Drug Treatment with Albendazole Sulfoxide and Albendazole Sulfone

Abstract : Alveolar echinococcosis (AE) is caused by the metacestode stage of the fox tapeworm Echinococcus mul-tilocularis. The disease affects the human liver and occasionally other organs and is fatal if treatment is unsuccessful. The present chemotherapy of AE is based on the administration of benzimidazole carbamate derivatives, such as mebendazole and albendazole. Albendazole treatment has been found to be ineffective in some cases, parasitostatic rather than parasiticidal, and the recurrence rate is rather high. Therefore, chemotherapy usually involves the lifelong uptake of massive doses of albendazole and new treatment options are urgently needed. In order to avoid costly and time-consuming animal experimentation, a first step in searching for novel parasiticidal compounds could be the in vitro drug screening of novel compounds by employing metacestode cultivation. However, presently used techniques (e.g., transmission electron micros-copy) for determination of parasite viability involve costly equipment and time-consuming preparation of rather large amounts of parasite material. We therefore searched for a parasite marker which can be easily traced and the presence or absence of which is indicative of parasite viability. In this study we show that the increase of E. multilocularis alkaline phosphatase activity in culture supernatants during in vitro drug treatment with albendazole derivatives correlates with the progressive degeneration and destruction of the meta-cestode tissue. The inexpensive and rapid assay presented here will serve as an ideal tool for performing first-round in vitro tests on the efficacy of a large number of antiparasitic compounds.
Complete list of metadatas

https://hal-univ-lyon1.archives-ouvertes.fr/hal-02110994
Contributor : Philippe Lawton <>
Submitted on : Friday, April 26, 2019 - 4:48:18 PM
Last modification on : Friday, April 26, 2019 - 5:16:21 PM

File

15-Stettler 01 modifié.pdf
Files produced by the author(s)

Identifiers

Collections

Citation

Marianne Stettler, M. Siles-Lucas, Elisabeth Sarciron, Philippe Lawton, Bruno Gottstein, et al.. Echinococcus multilocularis Alkaline Phosphatase as a Marker for Metacestode Damage Induced by In Vitro Drug Treatment with Albendazole Sulfoxide and Albendazole Sulfone. Antimicrobial Agents and Chemotherapy, American Society for Microbiology, 2001, 45 (8), pp.2256-2262. ⟨10.1128/AAC.45.8.2256-2262.2001⟩. ⟨hal-02110994⟩

Share

Metrics

Record views

152

Files downloads

25