Use of Percoll for the infection of cells in vitro with Cryptosporidium parvum oocysts

Abstract : A method for the infection of non-adherent THP-1 cells and adherent MDBK cells with Cryptosporidium parÕum oocysts using isotonic Percoll solutions was developed. Excystation was maximal after 2 h, but toxicity increased with the oocystrcell ratio and the incubation time. The infection rates did not increase with the oocystrcell ratio and both cell types were equally parasitized. q
Document type :
Journal articles
Complete list of metadatas

https://hal-univ-lyon1.archives-ouvertes.fr/hal-02110972
Contributor : Philippe Lawton <>
Submitted on : Friday, April 26, 2019 - 3:11:31 PM
Last modification on : Saturday, April 27, 2019 - 1:25:39 AM

File

14-Lawton 01 modifié.pdf
Files produced by the author(s)

Identifiers

Collections

Citation

Philippe Lawton, Roselyne Mancassola, Muriel Naciri, Anne-Françoise Pétavy. Use of Percoll for the infection of cells in vitro with Cryptosporidium parvum oocysts. Journal of Microbiological Methods, Elsevier, 2001, 46 (1), pp.81-84. ⟨10.1016/S0167-7012(01)00265-2⟩. ⟨hal-02110972⟩

Share

Metrics

Record views

21

Files downloads

26