Use of Percoll for the infection of cells in vitro with Cryptosporidium parvum oocysts

Philippe Lawton (1) , Roselyne Mancassola (2) , Muriel Naciri (2) , Anne-Françoise Pétavy (1)

1 UCBL ISPB - Université Claude Bernard Lyon 1 - Institut des Sciences Pharmaceutiques et Biologiques
2 PAP - Station de Pathologie aviaire et parasitologie [Nouzilly]

A method for the infection of non-adherent THP-1 cells and adherent MDBK cells with Cryptosporidium parÕum oocysts using isotonic Percoll solutions was developed. Excystation was maximal after 2 h, but toxicity increased with the oocystrcell ratio and the incubation time. The infection rates did not increase with the oocystrcell ratio and both cell types were equally parasitized. q

Mots clés: MDBK; Percoll; Cryptosporidium parvum; Culture; THP-1; In vitro

Domaines: Parasitologie

Philippe Lawton, Roselyne Mancassola, Muriel Naciri, Anne-Françoise Pétavy. Use of Percoll for the infection of cells in vitro with Cryptosporidium parvum oocysts. Journal of Microbiological Methods, 2001, 46 (1), pp.81-84. ⟨10.1016/S0167-7012(01)00265-2⟩. ⟨hal-02110972⟩