Use of a non-adherent cell culture system for testing the effect of 2',3'-dideoxyinosine against Cryptosporidium parvum

Philippe Lawton (1, 1) , Carine Hejl (2) , Marie-Elisabeth Sarciron (1) , Roselyne Mancassola (3) , Muriel Naciri (3) , Anne-Françoise Pétavy (1)

1 UCBL - Université Claude Bernard Lyon 1
2 UCBL ISPB - Université Claude Bernard Lyon 1 - Institut des Sciences Pharmaceutiques et Biologiques
3 PAP - Station de Pathologie aviaire et parasitologie [Nouzilly]

The in vitro cultivation of Cryptosporidium parvum in the non-adherent cell line THP-1 was evaluated for its capability as a useful additional model to investigate the effect of drugs on this parasite. The purine analog antiviral 2',3'-dideoxyinosine (ddI) was evaluated and compared to the reference molecule paromomycin in sequential 24 hour experiments beginning at 24 and 72 hour post-infection. The ability of this technique to evaluate the various parasite stages showed that ddI displayed a dose-dependent efficacy especially on the trophozoite and sexual stages. Paromomycin displayed a lower efficacy than previously reported. Both drugs induced a decrease in the number of multiparasitized cells. These results indicate that the purine salvage pathway should be a key chemotherapeutic target against C. parvum.

Mots clés: Cryptosporidium parvum; THP-1 cell; Paromomycin; 2' 3'-dideoxyinosine; In vitro

Domaines: Parasitologie; Chimie thérapeutique

Philippe Lawton, Carine Hejl, Marie-Elisabeth Sarciron, Roselyne Mancassola, Muriel Naciri, et al.. Use of a non-adherent cell culture system for testing the effect of 2',3'-dideoxyinosine against Cryptosporidium parvum. FEMS Microbiology Letters, 1999, 176 (1), pp.235-240. ⟨10.1111/j.1574-6968.1999.tb13667.x⟩. ⟨hal-02110959⟩