Effects of 2,3-Dideoxyinosine on Toxoplasma gondii Cysts in Mice

Marie-Elisabeth Sarciron 1 Philippe Lawton 2, 1 Charles Saccharin Anne-Françoise Pétavy 1 François Peyron 3
1 UCBL - Université Claude Bernard Lyon 1
2 Laboratoire de Parasitologie
3 Hôpital de la Croix-Rousse [CHU - HCL]
Abstract : The activity against Toxoplasma gondii of 2,3 dideoxyinosine (ddI), an anti-human immunodeficiency virus drug, was examined in an in vitro and in vivo study. Cell cultures infected with a strain known to cause chronic infections were used to show the dose-dependent effect of this drug compared with spiramycin and sulfadiazine. When a dose of 4 g/ml was used, no infected THP-1 cells or parasites were found after 60 h of incubation. An electron-microscopic study confirmed that after 12 h at 1 g/ml, the few parasites observed were severely altered. The treatment of chronically infected mice 3 months postinfection showed that a 30-day treatment with 2 mg of ddI/ml induced a significant reduction in the number of T. gondii cysts in the cerebral tissue. These cysts were not viable, as confirmed by immunofluorescence and reinfection experiments. These experiments suggest a possible role for ddI in the treatment of toxoplasmosis, and this possibility deserves further investigation.
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Marie-Elisabeth Sarciron, Philippe Lawton, Charles Saccharin, Anne-Françoise Pétavy, François Peyron. Effects of 2,3-Dideoxyinosine on Toxoplasma gondii Cysts in Mice. Antimicrobial Agents and Chemotherapy, American Society for Microbiology, 1997, 41 (7), pp.1531-1536. ⟨10.1128/AAC.41.7.1531⟩. ⟨hal-02110926⟩

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