In vitro cultivation of Cryptosporidium parvum in the non-adherent human monocytic THP-1 cell line

Philippe Lawton (1) , Muriel Naciri (2) , Roselyne Mancassola (2) , Anne-Francoise Pétavy (1)

1 UCBL - Université Claude Bernard Lyon 1
2 PAP - Station de Pathologie aviaire et parasitologie [Nouzilly]

This study shows that the human monocytic cell line THP-1 supports the growth of C. parvum. Immunofluorescence controls showed that only scarce oocysts remained after infection and disappeared Within the first 24 h of culture. A continuous asexual life cycle proceeded throughout the experiments, with at least 15d cultures. This model provides a useful tool for studies on the biology of C. parvum in cells involved in its transport in immunocompromised host

Domaines: Parasitologie; Biotechnologies

Philippe Lawton, Muriel Naciri, Roselyne Mancassola, Anne-Francoise Pétavy. In vitro cultivation of Cryptosporidium parvum in the non-adherent human monocytic THP-1 cell line. Journal of Eukaryotic Microbiology, 1997, 44 (s6), pp.66S. ⟨10.1111/j.1550-7408.1997.tb05783.x⟩. ⟨hal-02110909⟩