In vitro cultivation of Cryptosporidium parvum in the non-adherent human monocytic THP-1 cell line

Philippe Lawton 1 Muriel Naciri 2 Roselyne Mancassola 2 Anne-Francoise Pétavy 1
1 Laboratoire de Parasitologie
2 UEPAP - Unité Expérimentale Pathologie Aviaire et Parasitologie
Abstract : This study shows that the human monocytic cell line THP-1 supports the growth of C. parvum. Immunofluorescence controls showed that only scarce oocysts remained after infection and disappeared Within the first 24 h of culture. A continuous asexual life cycle proceeded throughout the experiments, with at least 15d cultures. This model provides a useful tool for studies on the biology of C. parvum in cells involved in its transport in immunocompromised host
Document type :
Journal articles
Complete list of metadatas
https://hal-univ-lyon1.archives-ouvertes.fr/hal-02110909
Contributor : Philippe Lawton <>
Submitted on : Friday, April 26, 2019 - 4:18:39 PM
Last modification on : Thursday, February 20, 2020 - 7:21:32 PM

File

9-Lawton 97 modifié.pdf
Files produced by the author(s)

Identifiers

Collections

UNIV-LYON1 | INRA | UDL

Citation

Philippe Lawton, Muriel Naciri, Roselyne Mancassola, Anne-Francoise Pétavy. In vitro cultivation of Cryptosporidium parvum in the non-adherent human monocytic THP-1 cell line. Journal of Eukaryotic Microbiology, Wiley, 1997, 44 (s6), pp.66S. ⟨10.1111/j.1550-7408.1997.tb05783.x⟩. ⟨hal-02110909⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

35

Files downloads

56

Contact

Informations

CCSD