In vitro cultivation of Cryptosporidium parvum in the non-adherent human monocytic THP-1 cell line

Philippe Lawton; Muriel Naciri; Roselyne Mancassola; Anne-Francoise Pétavy

In vitro cultivation of Cryptosporidium parvum in the non-adherent human monocytic THP-1 cell line

Philippe Lawton ¹ Muriel Naciri ² Roselyne Mancassola ² Anne-Francoise Pétavy ¹

2 UEPAP - Unité Expérimentale Pathologie Aviaire et Parasitologie

Abstract : This study shows that the human monocytic cell line THP-1 supports the growth of C. parvum. Immunofluorescence controls showed that only scarce oocysts remained after infection and disappeared Within the first 24 h of culture. A continuous asexual life cycle proceeded throughout the experiments, with at least 15d cultures. This model provides a useful tool for studies on the biology of C. parvum in cells involved in its transport in immunocompromised host

Document type :

Journal articles

Domain :

Life Sciences [q-bio] / Microbiology and Parasitology / Parasitology

Life Sciences [q-bio] / Biotechnology

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https://hal-univ-lyon1.archives-ouvertes.fr/hal-02110909
Contributor : Philippe Lawton <>
Submitted on : Friday, April 26, 2019 - 4:18:39 PM
Last modification on : Saturday, April 27, 2019 - 1:25:39 AM

9-Lawton 97 modifié.pdf

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In vitro cultivation of Cryptosporidium parvum in the non-adherent human monocytic THP-1 cell line

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In vitro cultivation of Cryptosporidium parvum in the non-adherent human monocytic THP-1 cell line

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