Skip to Main content Skip to Navigation
Journal articles

Considering external information to improve the phylogenetic comparison of microbial communities: a new approach based on constrained Double Principal Coordinates Analysis (cDPCoA)

Abstract : The use of next-generation sequencing technologies is revolutionizing microbial ecology by allowing a deep phylogenetic coverage of tens to thousands of samples simultaneously. Double Principal Coordinates Analysis (DPCoA) is a multivariate method, developed in community ecology, able to integrate a distance matrix describing differences among species (e.g. phylogenetic distances) in the analysis of a species abundance matrix. This ordination technique has been used recently to describe microbial communities taking into account phylogenetic relatedness. In this work, we extend DPCoA to integrate the information of external variables measured on communities. The constrained Double Principal Coordinates Analysis (cDPCoA) is able to enforce a priori classifications to retrieve subtle differences and (or) remove the effect of confounding factors. We describe the main principles of this new approach and demonstrate its usefulness by providing application examples based on published 16S rRNA gene data sets.
Document type :
Journal articles
Complete list of metadata

https://hal-univ-lyon1.archives-ouvertes.fr/hal-02044759
Contributor : Laure-Hélène Davoine Connect in order to contact the contributor
Submitted on : Thursday, February 21, 2019 - 4:18:28 PM
Last modification on : Wednesday, February 16, 2022 - 2:30:35 PM

Links full text

Identifiers

Collections

Citation

Stéphane Dray, S. Pavoine, D. Aguirre De Carcer. Considering external information to improve the phylogenetic comparison of microbial communities: a new approach based on constrained Double Principal Coordinates Analysis (cDPCoA). Molecular Ecology Resources, Wiley/Blackwell, 2015, 15 (2), pp.242-9. ⟨10.1111/1755-0998.12300⟩. ⟨hal-02044759⟩

Share

Metrics

Record views

17