Adipocytes, like their progenitors, contribute to inflammation of adipose tissues through promotion of Th-17 cells and activation of monocytes, in obese subjects - Université Claude Bernard Lyon 1 Accéder directement au contenu
Article Dans Une Revue Adipocyte Année : 2016

Adipocytes, like their progenitors, contribute to inflammation of adipose tissues through promotion of Th-17 cells and activation of monocytes, in obese subjects

M. Chehimi
  • Fonction : Auteur
M. Robert
  • Fonction : Auteur
M. E. Bechwaty
  • Fonction : Auteur
G. Vial
  • Fonction : Auteur
J. Rieusset
L. Pirola
A. Eljaafari

Résumé

Recently, we have reported that adipose tissue-derived stem cells (ASC) harvested from obese donors induce a pro-inflammatory environment when co-cultured with peripheral blood mononuclear cells (MNC), with a polarization of T cells toward the Th17 cell lineage, increased secretion of IL-1beta and IL-6 pro-inflammatory cytokines, and down-regulation of Th1 cytokines, such as IFNgamma and TNFalpha. However, whether differentiated adipocytes, like the aforementioned ASC, are pro-inflammatory in obese subject AT remained to be investigated. Herein, we isolated ASC from AT of obese donors and differentiated them into adipocytes, for either 8 or 14 d. We analyzed their capacity to activate blood MNC after stimulation with phytohemagglutinin A (PHA), or not, in co-culture assays. Our results showed that co-cultures of MNC with adipocytes, like with ASC, increased IL-17A, IL-1beta, and IL-6 pro-inflammatory cytokine secretion. Moreover, like ASC, adipocytes down-regulated TNFalpha secretion by Th1 cells. As adipocytes differentiated from ASC of lean donors also promoted IL-17A secretion by MNC, an experimental model of high-fat versus chow diet mice was used and supported that adipocytes from obese, but not lean AT, are able to mediate IL-17A secretion by PHA-activated MNCs. In conclusion, our results suggest that, as ASC, adipocytes in obese AT might contribute to the establishment of a low-grade chronic inflammation state.
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Dates et versions

hal-01850044 , version 1 (27-05-2020)

Identifiants

Citer

M. Chehimi, M. Robert, M. E. Bechwaty, G. Vial, J. Rieusset, et al.. Adipocytes, like their progenitors, contribute to inflammation of adipose tissues through promotion of Th-17 cells and activation of monocytes, in obese subjects. Adipocyte, 2016, 5 (3), pp.275-82. ⟨10.1080/21623945.2015.1134402⟩. ⟨hal-01850044⟩
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