Magnetic resonance imaging biomarkers of exercise-induced improvement of oxidative stress and inflammation in the brain of old high-fat-fed ApoE −/− mice

Abstract : Vascular brain lesions, present in advanced atherosclerosis, share pathological hallmarkswith p eripheral vascular lesions, such as increased inflammation and oxidative stress. Physicalactivity reduces these peripheral r isk factors, but its cerebrovascular effect is less documented,especially by non-invasive imaging . Through a combination of in vivo and post-mortemtechniques, we aimed to characterize vascular brain damage in old ApoE−/−mice fed ahigh -cholesterol (HC) diet with dietary controlled intake. We then sought to determine thebeneficial effects of exercise training on oxidative stress and inflammation in the brain as atreatment option in an ageing atherosclerosis mouse model. Using in vivo magnetic resonanceimaging (MRI) and biological markers of oxidative stress and inflammation, we evaluated theoccurrence of vascular abnormalities in the brain of HC-diet fed ApoE−/−mice >70 weeks old, itsassociation with local and systemic oxidative stress and inflammation, and whether both can b emodulated by exerc ise. Exercise t raining significantly reduced both MRI-detected abnormalities(present in 71% of untrained vs. 14% of trained mice) and oxidative stress (lipid peroxidation,9.1 ± 1.4 vs. 5.2 ± 0.9 µmol mg−1; P < 0.01) and inflammation (interleukin-1β, 226.8 ± 27.1vs. 182.5 ± 21.5 pg mg−1; P < 0.05) in the brain, and the mortality rate. Exercise also decreasedperipheral insulin resistance, oxidative stress and inflammation, but significant associations wereseen only within brain markers. Highly localized vascular brain damage is a frequent finding in this ageing athero sclerosis model, and exercise is able to reduce this outcome and improve lifespan.In vivo MRI evaluated both the neurovascular damage and the protective effect of exercise.
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Erica Chirico, Vanessa Di Cataldo, Fabien Chauveau, Alain Geloen, David Patsouris, et al.. Magnetic resonance imaging biomarkers of exercise-induced improvement of oxidative stress and inflammation in the brain of old high-fat-fed ApoE −/− mice. Journal of Physiology, Wiley, 2016, 594 (23), pp.6969 - 6985. ⟨10.1113/JP271903⟩. ⟨hal-01883914⟩

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