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Journal articles
A targeted functional RNA interference screen uncovers glypican 5 as an entry factor for hepatitis B and D viruses
Abstract : Chronic hepatitis B and D infections are major causes of liver disease and hepatocellular carcinoma worldwide. Efficient therapeutic approaches for cure are absent. Sharing the same envelope proteins, hepatitis B virus and hepatitis delta virus use the sodium/taurocholate cotransporting polypeptide (a bile acid transporter) as a receptor to enter hepatocytes. However, the detailed mechanisms of the viral entry process are still poorly understood. Here, we established a high-throughput infectious cell culture model enabling functional genomics of hepatitis delta virus entry and infection. Using a targeted RNA interference entry screen, we identified glypican 5 as a common host cell entry factor for hepatitis B and delta viruses. CONCLUSION: These findings advance our understanding of virus cell entry and open new avenues for curative therapies. As glypicans have been shown to play a role in the control of cell division and growth regulation, virus-glypican 5 interactions may also play a role in the pathogenesis of virus-induced liver disease and cancer
Keywords :
Viruses
Virus Internalization
Untranslated
therapy
RNA Interference
RNA
Proteins
Belgium
Liver
Infection
Humans
Hepatocytes
Hepatitis Delta Virus
pathogenicity
physiology
Medicine
Carcinoma
Cell Division
Cells
Culture
Cultured
France
Genomics
Germany
Glypicans
Growth
Hepatitis
Hepatitis B
Hepatitis B virus
https://hal-univ-lyon1.archives-ouvertes.fr/hal-01796197
Contributor : Lauriane Pillet <>
Submitted on : Saturday, May 19, 2018 - 11:34:31 AM
Last modification on : Thursday, November 26, 2020 - 3:56:10 PM
Contributor : Lauriane Pillet <>
Submitted on : Saturday, May 19, 2018 - 11:34:31 AM
Last modification on : Thursday, November 26, 2020 - 3:56:10 PM
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