A targeted functional RNA interference screen uncovers glypican 5 as an entry factor for hepatitis B and D viruses - Université Claude Bernard Lyon 1 Accéder directement au contenu
Article Dans Une Revue Hepatology Année : 2016

A targeted functional RNA interference screen uncovers glypican 5 as an entry factor for hepatitis B and D viruses

Eloi R. Verrier
Che C. Colpitts
  • Fonction : Auteur
Charlotte Bach
  • Fonction : Auteur
Mickaël Renaud
  • Fonction : Auteur
Sarah C. Durand
  • Fonction : Auteur
Georges Abou-Jaoudé
  • Fonction : Auteur
Maria M. López Ledesma
  • Fonction : Auteur
Daniel J. Felmlee
  • Fonction : Auteur
Magali Soumillon
  • Fonction : Auteur
Tom Croonenborghs
  • Fonction : Auteur
Nathalie Pochet
Mirjam B. Zeisel

Résumé

Chronic hepatitis B and D infections are major causes of liver disease and hepatocellular carcinoma worldwide. Efficient therapeutic approaches for cure are absent. Sharing the same envelope proteins, hepatitis B virus and hepatitis delta virus use the sodium/taurocholate cotransporting polypeptide (a bile acid transporter) as a receptor to enter hepatocytes. However, the detailed mechanisms of the viral entry process are still poorly understood. Here, we established a high-throughput infectious cell culture model enabling functional genomics of hepatitis delta virus entry and infection. Using a targeted RNA interference entry screen, we identified glypican 5 as a common host cell entry factor for hepatitis B and delta viruses. CONCLUSION: These findings advance our understanding of virus cell entry and open new avenues for curative therapies. As glypicans have been shown to play a role in the control of cell division and growth regulation, virus-glypican 5 interactions may also play a role in the pathogenesis of virus-induced liver disease and cancer

Dates et versions

hal-01796197 , version 1 (19-05-2018)

Identifiants

Citer

Eloi R. Verrier, Che C. Colpitts, Charlotte Bach, Laura Heydmann, Amélie Weiss, et al.. A targeted functional RNA interference screen uncovers glypican 5 as an entry factor for hepatitis B and D viruses. Hepatology, 2016, 63 (1), pp.35-48. ⟨10.1002/hep.28013⟩. ⟨hal-01796197⟩
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