Characterization of a novel canine T-cell line established from a spontaneously occurring aggressive T-cell lymphoma with large granular cell morphology
Résumé
Dogs with lymphoma are established as good model for human non-Hodgkin lymphoma studies. Canine cell lines derived from lymphomas may be valuable tools for testing new therapeutic drugs. In this context, we established a canine T-cell line, PER-VAS, from a primary aggressive T-cell lymphoma with large granular morphology. Flow cytometric analysis revealed a stable immunophenotype: PER-VAS cells were positively labelled for CD5, CD45, MHC II and TLR3, and were negative for CD3, CD4 and CD8 expression. Although unstable along the culture process, IL-17 and MMP12 proteins were detectable as late as at passages 280 and 325i.e. respectively 24 and 29 months post isolation. At passage 325, PER-VAS cells maintained the expression of IL-17, CD3, CD56, IFNgamma and TNFalpha mRNAs as shown by RT-PCR analysis. Stable rearrangement of the TCRgamma gene has been evidenced by PCR. PER-VAS cells have a high proliferation index with a doubling time of 16.5h and were tumorigenic in Nude mice. Compared to the canine cell lines already reported, PER-VAS cells display an original expression pattern, close to NKT cells, which makes them valuable tools for in vitro comparative research on lymphomas.
Mots clés
Interleukin-17/genetics/immunology
Lymphoma
Interferon-gamma/genetics/immunology
Male
Animals
RNA
Receptors
Antigen
Antigens
CD/genetics/immunology
CD56 mRNA
Cell line
Cell Line/*immunology/pathology
Dog
Dogs
Founder Effect
gamma-Chain T-Cell Antigen Receptor
gamma-delta/genetics/immunology
Gene Expression/*immunology
Gene Rearrangement
Il-17
Immunophenotyping
Humans
Mice
Messenger/genetics/*immunology
Nude
T-Cell
T-cell lymphoma
T-Cell/genetics/*immunology/pathology
T-Lymphocytes/*immunology/pathology
Toll-Like Receptor 3/genetics/immunology
Tumor Necrosis Factor-alpha/genetics/immunology
Domaines
Sciences du Vivant [q-bio]
Origine : Fichiers éditeurs autorisés sur une archive ouverte
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